Adv Pharm Bull. 2021;11(1): 86-95.
doi: 10.34172/apb.2021.009
  Abstract View: 525
  PDF Download: 324

Research Article

Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug

Asmaa Nabil Elboraey 1* ORCID logo, Hanan Hassan Abo-Almaged 2 ORCID logo, Ahmed Abd El-Rahman El-Ashmawy 3 ORCID logo, Aya Rashad Abdou 3 ORCID logo, Amani Ramadan Moussa 1 ORCID logo, Laila Hassanian Emara 3 ORCID logo, Hossam Mohammed El-Masry 4, Gehan El-Tabie El Bassyouni 2 ORCID logo, Magda Ismail Ramzy 1 ORCID logo

1 Fixed and Removable Prosthodontics Department, National Research Centre, 33 El Buhouth Street, Dokki, P.O.12622 Cairo, Egypt.
2 Refractories, Ceramics and Building Materials Department, National Research Centre, 33 El Buhouth Street, Dokki, P.O.12622, Cairo, Egypt.
3 Medicinal and Pharmaceutical Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, 33 EL Bohouth st. (former EL Tahrir st.), Dokki, Giza, Egypt, P.O.12622, Affiliation ID: 10014618.
4 Chemistry of Natural Microbial Products Department, National Research Centre,33 El Buhouth Street, Dokki, P.O.12622, Cairo, Egypt.
*Corresponding Author: Asmaa N. Elobraey, Tel: +02-33371635, Email: an.elboraey@nrc.sci.eg


Purpose: This study aimed to evaluate the biological and mechanical properties of the poly(methyl methacrylate) (PMMA) denture base material as a vehicle incorporating novel hydroxyapatite nanoparticles (HA-NP) loaded with metronidazole (MZ) drug.

Methods: HA-NP was prepared via wet-chemical-method, characterized by XRD, SEM/EDX, TEM, Fourier-transform infrared spectroscopy (FTIR), as well as the measurement of surface area and pore-size distribution. Four drug delivery formulas were prepared in the form of discs (10 x 2 mm) as follows: F1 (MZ/ HA-NP/PMMA), F2 (HA-NP/ PMMA), F3 (control-PMMA) and F4 (MZ/PMMA). Characterization of all formulas was performed using differential scanning calorimetry (DSC) and FTIR. MZ release rate, antimicrobial properties against three oral pathogens, cytotoxicity (MTT assay) and surface micro-hardness were also assessed. Statistical analysis of data was performed using one-way ANOVA test (P < 0.05).

Results: DSC thermograms showed compatibility among MZ, HA-NP and PMMA along with physical stability over 6 months storage period at room temperature. FTIR spectroscopy proved the absence of any possible chemical interaction with MZ. MZ-HA-NP/PMMA formula showed relatively better drug release compared to MZ-PMMA. Both formulas showed statistically significant antimicrobial potentials against two microbial strains. MTT demonstrated reduction in cell cytotoxicity after 96 hours with the least value for HA-NP. Surface micro-hardness revealed non-significant reduction compared with the control PMMA.

Conclusion: A novel biocompatible drug nanocarrier (HA-NP) was developed and incorporated in PMMA denture base material as a vehicle to allow prolonged sustained drug release to manage oral infections.

Keywords: PMMA discs, Nanocarriers, HP-NP, Drug delivery, Cytotoxicity, Surface micro-hardness
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Submitted: 07 Sep 2019
Revision: 22 Mar 2020
Accepted: 16 Apr 2020
ePublished: 07 Nov 2020
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