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Adv Pharm Bull. 2020;10(3): 437-443.
doi: 10.34172/apb.2020.053
  Abstract View: 66
  PDF Download: 43

Research Article

The Effect of Beta-Boswellic Acid on the Expression of Camk4 and Camk2α Genes in the PC12 Cell Line

Asiyeh Jebelli 1 ORCID logo, Mohammad Khalaj-Kondori 1 * ORCID logo, Mohammad Rahmati-Yamchi 2,3 * ORCID logo

1 Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
2 Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Purpose: Beta-boswellic acid (βBA) may play central roles in neural plasticity. Neural plasticityhas significant implications for learning and memory which are governed by strict memoryrelatedmolecular pathways. To gain insight into the molecular mechanism by which βBA affectsthese pathways this study analyzed the expression patterns of Camk2α and Camk4 genes inPC12 cells treated with βBA.Methods: The cytotoxic effects of different βBA concentrations on PC12 cells were examined byMTT assay. For gene expression analysis, cells were treated with concentrations of 1 and 10 μMof βBA for 12, 24, 48, and 72 hours. Total RNA was purified by RNX-Plus solution and reversetranscribed into cDNA using Thermo Scientific Reverse Transcription reagents. The expressionpatterns of Camk2α and Camk4 genes were quantified by quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR).Results: MTT assay indicated that βBA reduced PC12 cell viability in a time- and concentrationdependentmanner. The 50% inhibitory concentrations for the 48 and 72 hours time points were35 and 26 μM, respectively; while, the βBA concentrations up to 100 μM failed to kill 50% ofthe cells after 24 hours. According to the qRT-PCR data, the Camk2α variant is not expressed ineither βBA-treated or untreated PC12 cells. However, a significant upregulation was observedin Camk4 after 12 hours of treatment with βBA, which followed by a significant downregulationafter 24 hours and a persistent expression equal to the control until 72 hours.Conclusion: these findings indicate that PC12 cells not only does not express Camk2α but also itsexpression cannot be induced by βBA. However, βBA does modulate the expression of Camk4.This result provides further insight into the molecular mechanism by which βBA affects memory.
Keywords: Beta boswellic acid, Camk2α, Camk4, PC12 cells, Memory
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Submitted: 17 Sep 2019
Revision: 28 Nov 2019
Accepted: 08 Dec 2019
ePublished: 11 May 2020
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