Adv Pharm Bull. 2021;11(3): 469-476.
doi: 10.34172/apb.2021.054
  Abstract View: 261
  PDF Download: 15

Research Article

Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection

Rafael Souza Antunes ORCID logo, Douglas Vieira Thomaz ORCID logo, Luane Ferreira Garcia, Eric de Souza Gil, Flavio Marques Lopes* ORCID logo

1 Faculty of Pharmacy, Federal University of Goias, R. 240, S/n - Setor Leste Universitário, Goiânia - GO, Brazil. Zip Code: 74605-170.


Purpose: The development biosensing technologies capable of delivering fast and reliable analysis is a growing trend in drug quality control. Considering the emerging use of plant-based polyphenol oxidases (PPO) as biological component of electrochemical biosensors, this work reports the first Solanum lycocarpum PPO biosensor and its use in the pharmaceutical analysis of paracetamol in tablet formulations.
Methods: The biosensor was optimized regarding fruit maturation (immature and mature-ripe), vegetal extract volume to be used in biosensor construction as well as optimal pH of electrochemical cell fluid.
Results: Results evidenced that the extract which rendered the biosensor with best analytical performance was from immature fruits, and the biosensor produced using 100 µL of crude plant extract promoted better faradaic signal gathering. Moreover, when neutral pH media was used in the electrochemical cell, the biosensor showcased best faradaic signal output from the used redox probe (catechol), suggesting thence that the method presents high sensibility for phenolic compounds detection. Furthermore, the biosensor was able to quantify paracetamol in a linear range from 50 to 300 μM, showcasing LoD and LoQ of 3 μM and 10 μM, respectively.
Conclusion: after careful evaluation, this biosensor might be a low-cost alternative for conventional pharmaceutical quality control methods.
Keywords: Phenolic drugs, Biosensing technology, Enzymatic extract, Carbon paste electrode, Vegetal enzyme
First Name
Last Name
Email Address
Security code

Abstract View: 262

Your browser does not support the canvas element.

PDF Download: 15

Your browser does not support the canvas element.

Submitted: 15 Oct 2019
Revision: 30 Jun 2020
Accepted: 07 Jul 2020
ePublished: 07 Jul 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)