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Adv Pharm Bull. 2021;11(2): 378-384.
doi: 10.34172/apb.2021.036
  Abstract View: 448
  PDF Download: 104

Research Article

A Novel Approach on Leukodepletion Filters: Investigation of Synergistic Anticancer Effect of Purified α-Defensins and Nisin

Niloofar Sasani 1 ORCID logo, Rasoul Roghanian 1* ORCID logo, Giti Emtiazi 1, Afsaneh Aghaie 2

1 Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, P. O Box 81746-79441, Isfahan, Iran.
2 Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
*Corresponding Author: Rasoul Roghanian, Tel: +98 3137932457, Email: r.roghanian@sci.ui.ac.ir

Abstract

Purpose: There are number of reports available regarding defensins activity against mammalian cells besides their antimicrobial and immune regulatory activities. This study aims to investigate anticancer and apoptosis activity of the purified defensins from leukodepletion filters alone or in synergism with bacterial peptide, nisin, on prostate and colorectal cancer.

Methods: Leucoflex LCR-5 filters were backflushed by an optimized elution system. Isolated granulocytes were sonicated and the supernatant treated before further purification by high performance liquid chromatography (HPLC). SDS-PAGE and western blot testing verified the fraction. Cell culture on PC-3 (human prostate adenocarcinoma), and HCT-116 (human colorectal carcinoma) were conducted following by MTT assays in addition to annexin flow cytometry for sole and synergistic effects with peptide nisin.

Results: Viable and active neutrophils could recover, and α-defensins were extracted and purified. Combinations of an optimal dose of α-defensins and nisin showed a remarkable synergistic effect on cancer cell lines (over 90% and 70% for PC-3 and HCT-116, respectively).

Conclusion: It also observed that less than 40% of both cells could survive after co-treatment with optimal dose. Also, apoptosis was increased after treatment by these peptides together. Annexin Vpositive populations significantly increased in percentage in comparison with control.


Keywords: Defensin, Nisin, PC-3, HCT-116, Apoptosis
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Submitted: 23 Nov 2019
Revision: 09 Dec 2019
Accepted: 15 Apr 2020
ePublished: 19 Apr 2020
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