Adv Pharm Bull. 2021;11(3): 570-577.
doi: 10.34172/apb.2021.066
  Abstract View: 251
  PDF Download: 19

Research Article

Evaluation of Adjuvant Effectiveness of Alum-Propranolol Mixture on the Immunogenicity of Excreted/Secreted Antigens of Toxoplasma gondii RH Strain

Elyar Meshkini 1 ORCID logo, Arash Aminpour 2,1* ORCID logo, Khosrow Hazrati Tappeh 2,1, Shahram Seyyedi 3, Meysam Shokri 1

1 Department of Parasitology & Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
2 Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran
3 Department of Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.


Purpose: The introduction of novel adjuvants is an important step in attempts to develop a safe and more efficient vaccine. The present study was performed to determine whether the use of a mixed beta-adrenergic receptor antagonist propranolol (PRP) and aluminum (alum), as an adjuvant, have efficacy for Toxoplasma gondii (T. gondii) vaccine to induce protective immunity in a mouse model.
Methods: Female BALB/c mice divided into five groups were immunized with excretory-secretory antigens (ESA) vaccine, alum-ESA vaccine, PRP-ESA vaccine, and alum-PRP ESA vaccine, as well as with phosphate buffered saline (PBS), as a negative control group. The immune responses were evaluated by lymphocyte proliferation assay for measuring delayed-type hypersensitivity (DTH) response and by cytokine assay for evaluating IFN-γ and IL-5 levels. The survival rate of mice in all groups was assessed during a three-week monitoring period after an intraperitoneal challenge with T. gondii tachyzoites.
Results: The results showed that mice immunized with PRP, as an adjuvant, could secret a higher level of IFN-γ, which was significant in comparison to other groups. However, mice vaccinated with alum-precipitated ESA antigen had ability to produce an elevated level of IL-5 compared to other mouse groups (p≤0.05). Moreover, alum-PRP co-administration together with ESA vaccine resulted in the longer survival of mice.
Conclusions: The findings of this study revealed that the combination of alum-PRP adjuvants and ESA vaccine of T. gondii elicits both humoral and cellular immune responses, which are comparable to either alum or PRP alone.
Keywords: Toxoplasma gondii, Propranolol, Alum, Vaccine, Excreted/secreted antigens
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Abstract View: 252

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Submitted: 09 Dec 2019
Revision: 09 May 2020
Accepted: 07 Jul 2020
ePublished: 07 Jul 2020
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