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Adv Pharm Bull. 2021;11(1): 163-170.
doi: 10.34172/apb.2021.017
  Abstract View: 89
  PDF Download: 27

Research Article

Juxtaposition of Mesenchymal Stem Cells with Endothelial Progenitor Cells Promoted Angiogenic Potential Inside Alginate-Gelatin Microspheres

Shirin Saberianpour 1 ORCID logo, Reza Rahbarghazi 2,3* ORCID logo, Mahdi Ahmadi 2, Mohammad Nouri 2* ORCID logo, Morteza heidarzadeh 2, Abbas Karimi 1, Souror Nemati 4

1 Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Chemical Engineering Faculty, Sahand University of Technology, Tabriz, Iran.
*Corresponding Author: Reza Rahbarghazi and Mohammad Nouri; Email: and Email: nourimd@yahoo.com rahbarghazir@tbzmed.ac.ir

Abstract

Purpose: Here, we investigated the angiogenic potential of endothelial progenitor cells juxtaposed with mesenchymal stem cells (MSCs) inside alginate-gelatin microspheres with stromal derived factor-1α (SDF-1 α) for 7 days.

Methods: Six encapsulated groups were allocated including endothelial progenitor cells (EPCs), EPCs/SDF-1α, MSCs, MSCs/SDF-1α, EPCs+MSCs and EPCs+MSCs/SDF-1α. Cells were encapsulated with a mixture of 1% alginate and 2% gelatin hydrogel. Cell survival was examined by MTT assay. Endothelial differentiation was determined by flow cytometry and ELISA. Tubulogenesis assay and Ac-Dil-LDL uptake were used to detect functional activity. Cell migration was analyzed by Transwell insert and gelatin zymography analyses. By using real-time polymerase chain reaction (PCR), we measured the transcription of Akt and PK1.

Results: We found an increase in cell viability in MSCs/SDF-1α microspheres compared to EPCs group (P<0.05). EPC/MSCs co-culture contributed to the increase of CD133+ cells while we found high CD31 levels in MSCs group (P<0.05). Juxtaposition of EPC with MSCs increased tubulogenesis compared to SDF-1a-free condition (P<0.001). SDF-1α had the potential to increase in AC-LDL uptake in MSCs and EPCs/MSCs groups. Cells migration and MMP-9 activities increased after treatment with SDF-1α. SDF-1α upregulated PK1 and Akt in encapsulated cells, especially in a co-culture system.

Conclusion: Alginate-gelatin microspheres could alter the angiogenic potential of progenitor cells in the presence of SDF-1α

Keywords: Endothelial Progenitor Cells, Mesenchymal Stem Cells, Alginate-gelatin microspheres, Angiogenesis
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Submitted: 30 Jan 2020
Revision: 14 Mar 2020
Accepted: 19 Apr 2020
ePublished: 07 Nov 2020
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