Logo-apb
Adv Pharm Bull. 2021;11(2): 361-370.
doi: 10.34172/apb.2021.034
  Abstract View: 459
  PDF Download: 139

Research Article

In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus

Abouzar Babaei 1 ORCID logo, Hossein Bannazadeh Baghi 2,3 ORCID logo, Akram Nezhadi 4 ORCID logo, Zahra Jamalpoor 1* ORCID logo

1 Trauma Research Center, Aja University of Medical Sciences, Tehran, Iran.
2 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Neuroscience Research Center, Aja University of Medical Sciences, Tehran, Iran.
*Corresponding Author: Zahra Jamalpoor, Tel: +98-86096350; Email: z.jamalpoor@ajaums.ac.ir

Abstract

Purpose: Reovirus type 3 Dearing (ReoT3D), a wild type oncolytic virus (OV) from the Reoviridae family, kills KRAS mutant cancer cells. However, the use of OVs has faced with some limitations such as immune responses, and delivery of OVs to the tumor sites in systemic therapy. To solve this, and also to increase the anti-cancer effects of these OVs, mesenchymal stem cells (MSCs) might be used as an effective vehicle for OVs delivery. In this study, we examined the anti-cancer effects of human adipose derived-MSCs (AD-MSCs) as a vehicle of ReoT3D against human glioblastoma cells.

Methods: Here, AD-MSCs were characterized and toxicity of ReoT3D on them was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Then, capability of AD-MSCs for virus production was assessed by real-time polymerase chain reaction (PCR), and different in vitro anti-cancer experiments were applied for our anti-cancer purposes.

Results: Our results from toxicity assay revealed that the isolated and provoked AD-MSCs were resistant to nontoxic concentration multiplicity of infection (MOI) >1 pfu/cells of ReoT3D. In addition, the results indicated that AD-MSCs were susceptible for virus life cycle complementation and were capable for production of virus progenies. Furthermore, our results showed that AD-MSCs had oncolysis effects and increased the anti-cancer effects of ReoT3D.

Conclusion: AD-MSCs as a susceptible host for oncolytic reovirus could increase the anti-cancer activity of this OV against glioblastoma multiforme (GBM) cell line.


Keywords: Oncolytic virus, Reovirus type 3 Dearing, Mesenchymal stem cell, Glioblastoma cancer
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 460

Your browser does not support the canvas element.


PDF Download: 139

Your browser does not support the canvas element.

Submitted: 01 Mar 2020
Revision: 19 Apr 2020
Accepted: 20 Apr 2020
ePublished: 20 Apr 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)