Tayebeh Mahmoudi
1 
, Ali Jalili
2* , Kamal Abdolmohammadi
3, Shohreh Fakhari
2, Fatemeh Pahlavan
1, Ali Shekari
4, Bahram Nikkhoo
2, Lobat Tayebi
5, Mohammad Reza Rahmani
2* 1 Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2 Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
3 Department of Immunology, School of Medicine, Iranshahr University of Medical Sciences, Iranshahr, Iran.
4 Department of Basic Sciences, Farhangian University, Sanandaj, Iran.
5 Marquette University School of Dentistry, Milwaukee, WI, 53233, USA.
Abstract
Purpose: Acute pancreatitis (AP) which is distinguished by local pancreatic necrosis, followingby systemic organ failure is known as an inflammatory disease. Up to now, there are only a fewtreatment options accessible for patients suffering from AP. In this study, we aimed to examinethe anti-inflammatory capacities of human bone marrow-derived mesenchymal stromal cells(hBM-MSCs) in a detailed AP model experiment.Methods: AP was induced in C57BL/6 mice by intraperitoneal administration of cerulein (100μg/kg/h × 7 doses) at intervals of 1 hour. Then, 2×105 MSCs were infused in the AP mice bytail vein 6 hours after the last cerulein injection. Mice were sacrificed 12 hours following theinjection of hBM-MSC, and blood samples and pancreas tissues were obtained.Results: We first determined the presence of transplanted hBM-MSC in the pancreas of micewith AP, but not the control mice. Our data indicate that administration of hBM-MSCs to micewith AP lead to (i) decreased serum levels of amylase, lipase and myeloperoxidase activities, (ii)downregulation of proinflammatory cytokine, macrophage inflammatory protein 2 (MIP-2), and(iii) upregulation of the anti-inflammatory cytokine, interleukin 10 (IL-10). Moreover, hBM-MSCadministration results in notably attenuated cerulein-induced histopathological alternationsand edema.Conclusion: we demonstrate that hBM-MSC attenuates AP signs and indicating that hMB-MSCtherapy could be a suitable approach for the treatment of inflammatory disease such as AP.