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Adv Pharm Bull. 2021;11(4): 651-662.
doi: 10.34172/apb.2021.074
  Abstract View: 403
  PDF Download: 44

Research Article

Experimental Design Supported Liposomal Aztreonam Delivery: In Vitro Studies

Sayani Bhattacharyya 1* ORCID logo, Preethi Sudheer 1 ORCID logo, Kuntal Das 1 ORCID logo, Subhabrata Ray 2 ORCID logo

1 Krupanidhi College of Pharmacy, Bengaluru, Karnataka 560035, India.
2 Dr. BC Roy College of Pharmacy, Durgapur, West Bengal 713206, India.
*Corresponding Author: Sayani Bhattacharyya, Tel: +91-9845561865, Email: SayaniB@krupanidhi.edu.in

Abstract

Purpose: The present study focuses on a systemic approach to develop liposomal aztreonam as a promising dosage form for inhalation therapy in the treatment of pneumonia and explores the in-vitro antimicrobial and cell uptake efficacy.

Methods: Liposomes were prepared by ethanol injection method using the lipids - soya phosphatidylcholine (SP) and cholesterol (CH). A central composite design (CCD) was employed to optimize the lipid composition to evaluate the effect on vesicle size, zeta potential and entrapment efficiency of the formulation. A numerical and graphical optimization was carried out to predict the optimized blend. The optimized formulation was characterized for vesicle size, surface charge, encapsulation, surface morphology, differential scanning calorimetry (DSC), powder X Ray Diffraction (PXRD), thermogravimetric analysis (TGA), in vitro diffusion, accelerated stability studies, antimicrobial studies on Pseudomonas aeruginosa NCIM 2200 and in vitro cell uptake studies.

Results: The optimized formulation was found to have a particle size of 144 nm, a surface charge of -35 mV, with satisfactory drug entrapment. The surface morphology study proved the formation of nanosized vesicles. The drug release from liposomal matrix was biphasic in nature. The solid-state study revealed the reason for good encapsulation of drug. The moisture retention capacity was found to be minimum. The anti-microbial study revealed the potential antibacterial activity of the optimized formulation over the pure drug. The formulation was found to be safe on the epithelial cells and showed a marked increase in cellular uptake of aztreonam in a lipid carrier.

Conclusion: It can be concluded that the optimized liposomal aztreonam could be considered as a promising approach for the delivery of aztreonam through inhalation.


Keywords: Aztreonam, Liposomes, Statistical optimization, Antimicrobial study, Cell uptake study

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Submitted: 16 Jul 2020
Revision: 25 Aug 2020
Accepted: 15 Sep 2020
ePublished: 15 Sep 2020
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