Arezou Taghvimi
1 
, Fatemeh Soghra Jahed
2, Siavoush Dastmalchi
1,3, Yousef Javadzadeh
4* 1 Biotechnology Research Centre, Tabriz University of Medical Science, Tabriz, Iran.
2 Department of chemistry, Faculty of Science, Azarbijan Shahid Madani University, Tabriz, Iran.
3 Faculty of Pharmacy, Near East University, POBOX: 99138, Nicosia, North Cyprus, Mersin 10, Turkey.
4 Biotechnology Research Center, and Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
Abstract
Purpose: This research introduces a polymeric nanosphere as a new dispersive solid phaseextraction (DSPE) adsorbent for the extraction of methylphenidate (MPH) from urine and itshigh performance liquid chromatography (HPLC) analysis.Methods: Polymeric nanosphere is a kind of copolymeric network obtained by copolymerizationof an ionic liquid monomer and styrene in the presence of vinyltriethoxysilane and2-hydroxyethylmethacrylate. HPLC coupled with ultra violet detector was applied for thedetermination and quantification of MPH. Dominant parameters in extraction were modified bythe one-parameter-at-a-time method. The results are as follow: 10 mg of polymeric nanospheres(PNS), 400 μL of acetonitrile (ACT), 5 mL of urine with the pH value of 9, and the extractionand desorption times of 2 and 5 minutes, respectively, which can be selected as the optimumextraction conditions.Results: Calibration curve was plotted through optimized conditions, and the proposed methodwas validated. The results demonstrated that the method presented linearity in the concentrationrange of 30-1200 ng/mL. Selectivity, matrix effect and metabolites interference effect wereinvestigated and the method presented no obvious interference effect during the analysis runtime. Repeatability, limit of detection (LOD) and limit of quantification (LOQ) values of themethod can be reported in this section as well. The method showed satisfactory results with98.8% relative recovery in the analysis of positive urine samples.Conclusion: The findings convinced the applicability of the introduced method for DSPE andHPLC analysis of the positive urine samples in different laboratories.