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Adv Pharm Bull. 2023;13(1): 123-133.
doi: 10.34172/apb.2023.013
PMID: 36721809
PMCID: PMC9871267
  Abstract View: 984
  PDF Download: 357
  Full Text View: 93

Research Article

Surface Coating of Polyurethane Films with Gelatin, Aspirin and Heparin to Increase the Hemocompatibility of Artificial Vascular Grafts

Simzar Hosseinzadeh 1,2* ORCID logo, Forough Shams 3, Roya Fattahi 2, Ghader Nuoroozi 2, Elnaz rostami 4, lida Shahghasempour 5, Nasim Salehi-Nik 2* ORCID logo, Mahboubeh Bohlouli 2, Arash Khojasteh 2, Nazanin Ghasemi 6, Habibollah Peiravi 2

1 Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3 Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4 Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
5 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
6 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
*Corresponding Authors: *Corresponding Authors: Simzar Hosseinzadeh, Email: , Email: S.hosseinzadeh@sbmu.ac.ir; *Corresponding Authors: Nasim Salehi-Nik, Email: , Email: n.salehinik@gmail.com

Abstract

Purpose: A hemocompatible substrate can offer a wonderful facility for nitric oxide (NO) production by vascular endothelial cells in reaction to the inflammation following injuries. NO inhibits platelet aggregation this is especially critical in small-diameter vessels.

Methods: The substrate films were made of polyurethane (PU) in a casting process and after plasma treatments, their surface was chemically decorated with polyethylene glycol (PEG) 2000, gelatin, gelatin-aspirin, gelatin-heparin and gelatin-aspirin-heparin. The concentrations of these ingredients were optimized in order to achieve the biocompatible values and the resulting modifications were characterized by water contact angle and Fourier transform infra-red (FTIR) assays. The values of NO production and platelet adhesion were then examined.

Results: The water contact angle of the modified surface was reduced to 26±4⸰ and the newly developed hydrophilic chemical groups were confirmed by FTIR. The respective concentrations of 0.05 mg/ml and 100 mg/mL were found to be the IC50 values for aspirin and heparin. However, after the surface modification with aspirin, the bioactivity of the substrate increased in compared to the other experimental groups. In addition, there was a synergistic effect between these reagents for NO synthesis. While, heparin inhibited platelet adhesion more than aspirin.

Conclusion: Because of the highly hydrophilic nature of heparin, this reagent was hydrolyzed faster than aspirin and therefore its influence on platelet aggregation and cell growth was greater. Taken together, the results give the biocompatible concentrations of both biomolecules that are required for endothelial cell proliferation, NO synthesis and platelet adhesion.

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Submitted: 03 May 2021
Revision: 14 Oct 2021
Accepted: 31 Dec 2021
ePublished: 03 Jan 2022
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