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Adv Pharm Bull. 2024;14(1): 192-207.
doi: 10.34172/apb.2024.006
  Abstract View: 325
  PDF Download: 206

Research Article

Cobalt/Bioglass Nanoparticles Enhanced Dermal Regeneration in a 3-Layered Electrospun Scaffold

Zahra Hemmati Dezaki 1 ORCID logo, Kazem Parivar 1, Vahabodin Goodarzi 2 ORCID logo, Mohamad Reza Nourani 2* ORCID logo

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 Tissue Engineering and Regenerative Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
*Corresponding Author: Mohammad Reza Nourani, Emails: rnourani@bmsu.ac.ir, Email: r.nourani@yahoo.com

Abstract

Purpose: Due to the multilayered structure of the skin tissue, the architecture of its engineered scaffolds needs to be improved. In the present study, 45s5 bioglass nanoparticles were selected to induce fibroblast proliferation and their protein secretion, although cobalt ions were added to increase their potency.

Methods: A 3-layer scaffold was designed as polyurethane (PU) - polycaprolactone (PCL)/ collagen/nanoparticles-PCL/collagen. The scaffolds examined by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), tensile, surface hydrophilicity and weight loss. Biological tests were performed to assess cell survival, adhesion and the pattern of gene expression.

Results: The mechanical assay showed the highest young modulus for the scaffold with the doped nanoparticles and the water contact angle of this scaffold after chemical crosslinking of collagen was reduced to 52.34±7.7°. In both assessments, the values were statistically compared to other groups. The weight loss of the corresponding scaffold was the highest value of 82.35±4.3 % due to the alkaline effect of metal ions and indicated significant relations in contrast to the scaffold with non-doped particles and bare one (P value<0.05). Moreover, better cell expansion, greater cell confluence and a lower degree of toxicity were confirmed. The up-regulation of TGF β1 and VEGF genes introduced this scaffold as a better model for the fibroblasts commitment to a new skin tissue among bare and nondoped scaffold (P value<0.05).

Conclusion: The 3-layered scaffold which is loaded with cobalt ions-bonded bioglass nanoparticles, is a better substrate for the culture of the fibroblasts.

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Submitted: 10 Mar 2022
Revision: 12 Nov 2022
Accepted: 19 Jul 2023
ePublished: 22 Jul 2023
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