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Adv Pharm Bull. 2024;14(2): 378-387.
doi: 10.34172/apb.2024.025
PMID: 39206390
PMCID: PMC11347738
  Abstract View: 342
  PDF Download: 196

Research Article

Fabrication of Anti-glaucoma Nanofibers as Controlled-Release Inserts for Ophthalmic Delivery of Brimonidine Tartrate: In Vivo Evaluation in Caprine Eye

Fariba Shaikhi Shoushtari 1 ORCID logo, Mohammadshakib Naghshbandy 1, Leila Rezaei 2, Saba Mehrandish 3 ORCID logo, Shahla Mirzaeei 4,5* ORCID logo

1 Department of Ophthalmology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2 Department of Ophthalmology, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3 Pharmaceutical Sciences Research Center, Rahesh Daru Novine, Kermanshah 6715847141, Iran.
4 Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
5 Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
*Corresponding Author: Shahla Mirzaeei, Email: smirzaeei@kums.ac.ir, Email: shahlamirzaeei@gmail.com

Abstract

Purpose: Chronic ailments usually decrease the quality of life due to the requirement for repetitive administration of drugs. Glaucoma is a chronic eye disease occurred because of increased intraocular pressure (IOP). Controlled-release inserts can overcome this challenge by a gradual release of the antiglaucoma drugs. This study aimed to fabricate ocular inserts of brimonidine tartrate (BMD) for the management of glaucoma.

Methods: Different polymers including poly (D, L-lactide), polycaprolactone, cellulose acetate, and Eudragit RL100® were used to develop the BMD-loaded nanofibrous inserts by electrospinning technique. The inserts were characterized. The morphology and drug-polymer compatibility were examined by scanning electron microscopy (SEM), and Fourier-transform infrared (FTIR) spectroscopy and in vitro drug release in PBS. The IOP-lowering efficacy and irritancy of optimized formulation were assessed in the caprines.

Results: SEM images demonstrated nanofibers with uniform morphology and a mean diameter<300 nm were fabricated. The nanofibers were high-strength and flexible enough to be placed in the conjunctival sac. FTIR showed drug-polymer compatibility. In vitro release study indicated a sustained-release profile of the drug during 6 days for inserts. In vivo evaluation indicated that the optimized formulation is capable of maintaining the IOP in a non-glaucomatous range for an extended duration of 6 days. In addition, the formulation was non-irritant to the caprine eye.

Conclusion: Due to the prolonged IOP-lowering efficiency, BMD-loaded nanofibrous inserts can be considered suitable for the controlled release of drugs and thus enhance patient compliance by reducing the frequency of administration.

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Submitted: 04 Aug 2022
Revision: 02 Aug 2023
Accepted: 07 Jan 2024
ePublished: 13 Jan 2024
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