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Submitted: 25 Jul 2024
Revision: 22 Feb 2025
Accepted: 05 Mar 2025
ePublished: 08 Mar 2025
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Adv Pharm Bull. 2025;15(1): 206-216.
doi: 10.34172/apb.43476
  Abstract View: 141
  PDF Download: 32

Original Article

Well-regulated Dermal Regeneration Using Amnion-containing Scaffold in a Preclinical Study

Masumeh Staji 1 ORCID logo, Arezoo Karamivandishi 1 ORCID logo, Roya Fattahi 2, Masoud Soleimani 1,3, Hamidreza Moosavian 4, Simzar Hosseinzadeh 1,3* ORCID logo

1 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2 Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
3 Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4 Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
*Corresponding Author: Simzar Hosseinzadeh, Email: symzar.hosseinzadeh@gmail.com

Abstract

Purpose: we investigated the synergistic influence of amnion and keratinocytes on dermal regeneration in mice.

Methods: A scaffold derived from amnion and gelatin via electrospinning was used to synthesize a polyurethane-based scaffold. polyurethane/gelatin/amnion (PU/G/A) scaffold was characterized by scanning electron microscopy (SEM), FTIR, and tensile test. biocompatibility of the corresponding scaffold was investigated using the MTT method in the culture of keratinocytes.

Results: The SEM images showed sufficient cell adhesion on the PU/G/A scaffold. The tensile test results indicated that the scaffold containing PU/G/A with the lowest Young’s modulus (12 MPa±2.1) displayed higher elasticity than the scaffold without amnion. Furthermore, the MTT assay revealed that the amniotic scaffold contributed to 100% cell viability (P≤0.0001 compared to control) and proliferation. Moreover, an in vivo study conducted on mice showed that the PU/G/A/ keratinocytes scaffold results in increased granulation, tissue formation and wound closure (P<0.001 compared to control).

Conclusion: This innovative nanofiber device not only addresses the limitations of traditional dressings but also offers additional functionalities such as wound compatibility, gas exchange, promotion of angiogenesis in the injured area and a substrate that amplifies the biological functions of stem cells.


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