Abstract View: 88

Research Article

A Novel Approach on Leukodepletion Filters: Investigation of Synergistic Anticancer Effect of Purified α-Defensins and Nisin

Niloofar Sasani ORCID logo, Rasoul Roghanian* ORCID logo, Giti Emtiazi, Afsaneh Aghaie


Introduction: There are numbers of reports available regarding defensins activity against mammalian cells besides their antimicrobial and immune regulatory activities. This study aims to investigate anticancer and apoptosis activity of the purified defensins from leukodepletion filters alone or in synergism with bacterial peptide, nisin, on prostate and colorectal cancer. Methods: Leukoflex LCR-5 filters were back- flushed by an optimized elution system. Isolated granulocytes were sonicated and the supernatant treated prior to further purification by HPLC. The fraction was verified by SDS-PAGE and western blot testing. Cell culture on PC-3 (human prostate adernocarcinoma) and HCT-116 (human colorectal carcinoma) was conducted following by MTT assays in addition to annexin flow cytometry for sole and synergistic effects with peptide nisin. Results: Viable and active neutrophils could be recovered and α-defensins were extracted and purified. Combinations of optimal dose of α-defensins and nisin showed a remarkable synergistic effect on cancer cell lines (over 90% and 70% for PC-3 and HCT-116 respectively). Conclusion: It was also observed that less than 40% of both cells could be survived after co-treatment with optimal dose. Also apoptosis was increased after treatment by these peptides together. Annexin V positive populations were significantly increased in percentage in comparison with control.
Keywords: Defensin, Nisin, Apoptosis, Leukodepletion Filters, PC-3, HCT-116
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Abstract View: 88

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Submitted: 23 Nov 2019
Revision: 11 Mar 2020
Accepted: 15 Apr 2020
ePublished: 19 Apr 2020
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