Logo-apb
  Abstract View: 75

Research Article

Humanized Culture Medium for Clinical-grade Generation of Erythroid Cells from Umbilical Cord Blood CD34+ Cells

Majid Zamani 1,2 ORCID logo, Yoda Yaghoubi 2,3, Adel Naimi 4, Ali Hassanzadeh 5, Ramin Pourakbari 6,7, Leili Aghebati-Maleki 1, Roza Motavalli 6, Afsoon Aghlmandi 6, Amir Mehdizadeh 8, Mehdi Nazari 9, Mehdi Yousefi 6,7 ORCID logo, Ali Akbar Movassaghpour 10* ORCID logo

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Medical Laboratory Sciences, Faculty of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran.
4 Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
5 Department of Tissue Engineering and Applied Cell Sciences, Tehran University of Medical Sciences, Tehran, Iran.
6 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
7 Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran.
8 Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
9 Department of Anesthesiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
10 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost.
Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and Glycophorin A expression levels were evaluated.
Results: Maximum HSCs proliferation was observed on day 15 in PL-containing medium (99±17×103-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium.
Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ hematopoietic stem cells (HSCs). PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation.
Keywords: Human Platelet Lysate, Fetal Bovine Serum, CD34+ Hematopoietic Stem Cells, Erythroid Differentiation
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 75

Your browser does not support the canvas element.


PDF Download: 0

Your browser does not support the canvas element.

Submitted: 21 Jan 2020
Revision: 03 Feb 2020
Accepted: 29 Feb 2020
ePublished: 29 Feb 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)