Majid Zamani
1 
, Yoda Yaghoubi
2, Adel Naimi
3, Ali Hassanzadeh
4, Ramin Pourakbari
2, Leili Aghebati-Maleki
5, Roza Motavalli
2, Afsoon Aghlmandi
2, Amir Mehdizadeh
6, Mehdi Nazari
7, Mehdi Yousefi
2 , Ali Akbar Movassaghpour
5,8* 1 Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
2 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
4 Department of Tissue Engineering and Applied Cell Sciences, Tehran University of Medical Sciences, Tehran, Iran.
5 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
7 Department of Anesthesiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
8 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
9 .
10 .
Abstract
Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost.
Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated.
Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99±17×103 -fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium.
Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation.