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  Abstract View: 62

Research Article

In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus

Abouzar Babaei 1 ORCID logo, Hossein Bannazadeh Baghi 2,3 ORCID logo, Akram Nezhadi 4 ORCID logo, Zahra Jamalpoor 1* ORCID logo

1 Trauma Research Center, Aja University of Medical Sciences, Tehran, Iran.
2 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Neuroscience Research Center, Aja University of Medical Sciences, Tehran, Iran.

Abstract

Purpose: Reovirus type 3 Dearing (ReoT3D), a wild type oncolytic virus (OV) from the Reoviridae family, kills KRAS mutant cancer cells. However, the use of oncolytic viruses (OVs) has faced with some limitations such as immune responses, and delivery of OVs to the tumor sites in systemic therapy. To solve this, and also to increase the anti-cancer effects of these OVs, mesenchymal stem cells (MSCs) might be used as an effective vehicle for OVs delivery. In this study, we examined the anti-cancer effects of human Adipose Derived-MSCs (AD-MSCs) as a vehicle of ReoT3D against human gliobastoma cells.
Methods: Here, AD-MSCs were characterized and toxicity of ReoT3D on them was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Then, capability of AD-MSCs for virus production was assessed by real-time PCR, and different in vitro anti-cancer experiments were applied for our anti-cancer purposes.
Results: Our results from toxicity assay revealed that the isolated and provoked AD-MSCs were resistant to nontoxic concentration multiplicity of infection (MOI) >1 pfu/cells of ReoT3D. In addition, the results indicated that AD-MSCs were susceptible for virus life cycle complementation and were capable for production of virus progenies. Furthermore, our results showed that AD-MSCs had oncolysis effects and increased the anti-cancer effects of ReoT3D.
Conclusion: AD-MSCs as a susceptible host for oncolytic reovirus could increase the anti-cancer activity of this OV against glioblastoma multiforme (GBM) cell line.
Keywords: Oncolytic virus, Reovirus type 3 Dearing, Mesenchymal stem cell, Glioblastoma cancer
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Submitted: 01 Mar 2020
Revision: 19 Apr 2020
Accepted: 20 Apr 2020
ePublished: 20 Apr 2020
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