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Adv Pharm Bull. 2015;5(5): 621-627. doi: 10.15171/apb.2015.084
PMID: 26793607        PMCID: PMC4708032

Original Research

Chaperone-Assisted Soluble Expression of a Humanized Anti-EGFR ScFv Antibody in E. Coli

Kamal Veisi 1,2,3, Safar Farajnia 1,4 * , Nosratollah Zarghami 2, Hamid Reza Khoram Khorshid 5, Nasser Samadi 4,2, Shiva Ahdi Khosroshahi 4, Hossein Zarei Jaliani 6

Cited by CrossRef: 11


1- Ravitchandirane G, Bandhu S, Chaudhuri T. Multimodal approaches for the improvement of the cellular folding of a recombinant iron regulatory protein in E. coli. Microb Cell Fact. 2022;21(1) [Crossref]
2- Farajnia S, Ghorbanzadeh V, Dariushnejad H. Effect of Molecular Chaperone on the Soluble Expression of Recombinant Fab Fragment in E. coli. Int J Pept Res Ther. 2020;26(1):251 [Crossref]
3- Liu C, Kobashigawa Y, Yamauchi S, Toyota Y, Teramoto M, Ikeguchi Y, Fukuda N, Sato T, Sato Y, Kimura H, Morioka H. Preparation of single-chain Fv antibodies in the cytoplasm of Escherichia coli by simplified and systematic chaperone optimization. 2019;166(6):455 [Crossref]
4- Rodrigues J, Rodrigues L. Potential Applications of the Escherichia coli Heat Shock Response in Synthetic Biology. Trends in Biotechnology. 2018;36(2):186 [Crossref]
5- Banisadr A, Safdari Y, Kianmehr A, Pourafshar M. Production of a germline-humanized cetuximab scFv and evaluation of its activity in recognizing EGFR- overexpressing cancer cells. Human Vaccines & Immunotherapeutics. 2018;14(4):856 [Crossref]
6- Yousefi M, Dadashpour M, Hejazi M, Hasanzadeh M, Behnam B, de la Guardia M, Shadjou N, Mokhtarzadeh A. Anti-bacterial activity of graphene oxide as a new weapon nanomaterial to combat multidrug-resistance bacteria. Materials Science and Engineering: C. 2017;74:568 [Crossref]
7- Mamipour M, Yousefi M, Hasanzadeh M. An overview on molecular chaperones enhancing solubility of expressed recombinant proteins with correct folding. International Journal of Biological Macromolecules. 2017;102:367 [Crossref]
8- Sandomenico A, Sivaccumar J, Ruvo M. Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments. IJMS. 2020;21(17):6324 [Crossref]
9- Fatima K, Naqvi F, Younas H. A Review: Molecular Chaperone-mediated Folding, Unfolding and Disaggregation of Expressed Recombinant Proteins. Cell Biochem Biophys. 2021;79(2):153 [Crossref]
10- Lotfi H, Sheervalilou R, Zarghami N. An update of the recombinant protein expression systems of Cyanovirin-N and challenges of preclinical development. BI. 2017;8(2):139 [Crossref]
11- Yurkova M, Fedorov A. GroEL—A Versatile Chaperone for Engineering and a Plethora of Applications. Biomolecules. 2022;12(5):607 [Crossref]