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Adv Pharm Bull. 2016;6(2): 187-194. doi: 10.15171/apb.2016.026
PMID: 27478780        PMCID: PMC4961976

Research Article

Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System

Abbas Mohajeri 1, Yones Pilehvar-Soltanahmadi 1,2, Mohammad Pourhassan-Moghaddam 2, Jalal Abdolalizadeh 3, Pouran Karimi 4, Nosratollah Zarghami 1,2 *

Cited by CrossRef: 9


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2- Zhao Z, Yuwen W, Zhu W, Qu L, Fan D. Signal Peptide-Guided Delivery of a Mucin-Like Collagen Analogue for Periplasmic Barrier Reinforcement: A Platform for Enhancing Microbial Survival. ACS Synth Biol. 2025;14(9):3767 [Crossref]
3- Li M, Wang Z, Zhou M, Zhang C, Zhi K, Liu S, Sun X, Wang Z, Liu J, Liu D. Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm. Front Microbiol. 2022;13 [Crossref]
4- Yadollahi N, Mohamadian T, Esmaeili D, Forohi F. Design of recombinant bacteriocin fusion protein and evaluation of its anticancer and antibacterial activity. Microbial Pathogenesis. 2025;205:107633 [Crossref]
5- Shan Y, Wang B, Zhang J. New strategies in achieving antiangiogenic effect: Multiplex inhibitors suppressing compensatory activations of RTKs. Medicinal Research Reviews. 2018;38(5):1674 [Crossref]
6- Valipour A, Namdar Ahmad Abad H, Vatanchian M, Arezumand R. Designing and Developing Enzyme-Linked Immunosorbent Assay Sandwich Kit for Measuring Placental Growth Factor Concentration. Monoclonal Antibodies in Immunodiagnosis & Immunotherapy. 2023;42(1):3 [Crossref]
7- Liu Z, Chen H, Zheng L, Sun L, Shi L. Angiogenic signaling pathways and anti-angiogenic therapy for cancer. Sig Transduct Target Ther. 2023;8(1) [Crossref]
8- Mohajeri A, Abdolalizadeh J, Pilehvar-Soltanahmadi Y, Kiafar F, Zarghami N. Expression and Secretion of Endostar Protein by Escherichia Coli: Optimization of Culture Conditions Using the Response Surface Methodology. Mol Biotechnol. 2016;58(10):634 [Crossref]
9- Romero-Martínez D, Gasteazoro F, Cofas-Vargas L, García‑Hernández E, González-Davis O, Zavala G, Rosiles-Becerril D, Vazquez-Duhalt R, Trujillo‑Roldán M, Valdez‑Cruz N. Catalytically active inclusion bodies of Bacillus clausii laccase protein recombinantly produced in E. coli for dye decolorization. Microb Cell Fact. 2026; [Crossref]
10- Tan J, Ding N, Zhang X, Li W, Lin Y, Wang Q, Li J, Wu Q, Hu X, Jiang G, Zheng Y. Extracellular production of N-glycosylated Fn3 in Escherichia coli via optimized signal peptides, strains, and chemical reagents. International Journal of Biological Macromolecules. 2025;320:145778 [Crossref]
11- Dai Y, Yao Y, He Y, Hu X. Role of vascular endothelium and exosomes in cancer progression and therapy (Review). Int J Oncol. 2024;66(1) [Crossref]
12- Santos B, Morones-Ramirez J, Balderas-Renteria I, Casillas-Vega N, Galbraith D, Zarate X. Optimizing Periplasmic Expression in Escherichia coli for the Production of Recombinant Proteins Tagged with the Small Metal-Binding Protein SmbP. Mol Biotechnol. 2019;61(6):451 [Crossref]
13- Saman H, Raza S, Uddin S, Rasul K. Inducing Angiogenesis, a Key Step in Cancer Vascularization, and Treatment Approaches. Cancers. 2020;12(5):1172 [Crossref]