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EBSCOhost; CAS: DOAJ

Adv Pharm Bull. 2020;10(4): 610-616. doi: 10.34172/apb.2020.073

PMID: 33072539 PMCID: PMC7539321

Research Article

Improving the Expression of Human Granulocyte Colony Stimulating Factor in Escherichia coli by Reducing the GC-content and Increasing mRNA Folding Free Energy at 5’-Terminal End

Kartika Sari Dewi ¹ * , Asrul Muhamad Fuad ¹

Cited by CrossRef: 6

1- Nuryana I, Laksmi F, Agustriana E, Dewi K, Andriani A, Thontowi A, Kusharyoto W, Lisdiyanti P. Expression of Codon-Optimized Gene Encoding Murine Moloney Leukemia Virus Reverse Transcriptase in Escherichia coli. Protein J. 2022;41(4-5):515 [Crossref]

2- Song Y, Wang Y, Yan S, Nakamura K, Kikukawa T, Ayabe T, Aizawa T. Efficient recombinant production of mouse-derived cryptdin family peptides by a novel facilitation strategy for inclusion body formation. Microb Cell Fact. 2023;22(1) [Crossref]

3- Paremskaia A, Kogan A, Murashkina A, Naumova D, Satish A, Abramov I, Feoktistova S, Mityaeva O, Deviatkin A, Volchkov P. Codon-optimization in gene therapy: promises, prospects and challenges. Front Bioeng Biotechnol. 2024;12 [Crossref]

4- Laksmi F, Dewi K, Nuryana I, Yulianti S, Ramadhan K, Hadi M, Nugraha Y. High-level expression of codon-optimized Taq DNA polymerase under the control of rhaBAD promoter. Analytical Biochemistry. 2024;692:115581 [Crossref]

5- Wang G, Kuo J, Cheng C, Chung Y. Single-Chamber Microbial Fuel Cell with an Innovative Sensing Component for Real-Time Continual Monitoring of a Wide Range of Cr(VI) Concentrations in Wastewater. Biosensors. 2025;15(3):158 [Crossref]

6- Dewi K, Tasia W, Agustiyanti D, Hariyatun H, Wisnuwardhani P, Rubiyana Y, Arifa N, Purba H, Forentin A, Utami R, Kusharyoto W, Wardiana A. Soluble Expression of Recombinant Human Mitsugumin 53 in Escherichia coli NiCo21(DE3). Protein J. 2026; [Crossref]

7- Dewi K, Wahyuni F, Salsabila S, Salsabila A, Yanthi N, Fuad A. Expression comparison between two genes encoding CSF3 recombinant proteins having different codon composition at N-terminal in Escherichia coli . IOP Conf Ser: Earth Environ Sci. 2021;948(1):012081 [Crossref]

8- Zabolotskii A, Riabkova N. A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli. Biotechnology Journal. 2024;19(4) [Crossref]