Adv Pharm Bull. 2018;8(4):683-695.
doi: 10.15171/apb.2018.077
  Abstract View: 17
  PDF Download: 41

Research Article

Molecular Identification and Probiotic Potential Characterization of Lactic Acid Bacteria Isolated from Human Vaginal Microbiota

Abstract

Purpose: The increased demand for probiotics because of their health purposes provides the context for this study, which involves the molecular identification of LAB obtained from the vaginal microbiota of healthy fertile women. The isolates were subjected for examination to prove their probiotic potential. In particular, the isolates were subjected to various tests, including acid/bile tolerance, antimicrobial activity, antibiotic susceptibility, gram staining, and catalase enzyme activity assessment. Methods: Several methods were utilized for the molecular identification of the isolates, including ARDRA, (GTG)5-PCR fingerprinting, and the PCR sequencing of 16S-rDNA amplified fragments. Disc diffusion and well diffusion methods were used to assess antibiotic susceptibility and antibacterial activity of isolates. Tolerance to acid and bile was performed at pH 2.5 and 0.3% bile oxgall. Results: A total of 45 isolates of 88 separate organisms was selected. All of the isolates demonstrated an antibacterial effect on the exploited indicator microorganisms. All selected strains also maintained their viability at low-pH and high-bile conditions and exhibited abroad variation in their survival. Only the Enterococcus avium strain showed resistance to all 9 tested antibiotics. Based on the molecular identification and clustering, the 45 isolated bacteria were classified into three major groups of LAB: enterococci, lactobacilli and lactococci. Conclusions: Lactic acid bacteria (LAB) are microorganisms that have a particularly important function in maintaining the health of the vaginal and gastrointestinal tract and in protecting it from infection by other pathogenic organisms. The isolates found to be a promising probiotic candidate by showed desirable characteristics, acid and bile tolerance. Therefore, strain DL3 can be used as natural food preservative with some more potential investigations.
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Submitted: 22 Nov 2017
Revised: 28 Jul 2018
Accepted: 15 Aug 2018
First published online: 16 Aug 2018
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