Adv Pharm Bull. 2018;8(2):277-282.
doi: 10.15171/apb.2018.032
  Abstract View: 217
  PDF Download: 272

Research Article

PTPN22 Silencing in Human Acute T-Cell Leukemia Cell Line (Jurkat Cell) and its Effect on the Expression of miR-181a and miR-181b

Elham Baghbani 1, Vahid Khaze 1, Sanam Sadreddini 1, Ahad Mokhtarzadeh 1, Behzad Mansoori 1, Ali Mohammadi 1, Vida Vatankhahan 1, Parisa Toosi 1, Behzad Baradaran 1 *

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.


Purpose: T-cell acute lymphoblastic leukemia (T-ALL) is one of the most common malignancies associated with T-lymphocytes, accounting for 10 to 15 percent of ALL cases in children and 25 percent in adults. Innovative therapeutic approaches that overcome ineffective treatments on tumor cells may be a potential source of improvement in therapeutic approaches. Suppression of gene expression at transfusion level is one of the important strategies in gene therapy. The expression of PTPN22 and miR-181 genes in all types of hematologic malignancies increases and is likely to contribute to the survival and death of cells by affecting a variety of signaling pathways. The purpose of this study was to determine the role of PTPN22 inhibition by siRNA, and alteration in miR-181a and miR-181b in Jurkat cell line. Methods: Jurkat cells were transfected with 80 pmol of siRNA to inhibit PTPN22. After that, expression of PTPN22 mRNA and transcript levels of miR-181a and miR-181b were measured with Real-time PCR after 48hrs. Results: Experiments demonstrated that siRNA transfection resulted in significant downregulation of PTPN22 mRNA after 48 hrs in 80 pmol dose of siRNA. Moreover, transcript levels of both miR-181a and miR-181b was decreased after transfection. Conclusion: PTPN22, miR-181a and miR-181b might be involved in progression of Jurkat cells and targeting these molecules by RNAi might confer promising tool in treatment of T-ALL.
Keywords: T-ALL, PTPN22, miR-181a, miR-181b
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Submitted: 14 Mar 2018
Revised: 12 Apr 2018
Accepted: 17 May 2018
First published online: 19 Jun 2018
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