Abstract
Purpose:
Ofloxacin is a fluoroquinolone
with broad-spectrum antibacterial action, used in treatment of systemic and
local infections. Ofloxacin is BCS class II drug having low solubility, high
permeability with short half-life. The present work was aimed to design,
develop and optimize gellified emulsion of Ofloxacin to provide site targeted
drug delivery. Transdermal drug delivery will enhance the bioavailability of
the drug giving controlled drug release.
Methods:
Transdermal drug delivery system
was designed with gelling agent (Carbopol 940 and HPMC K100M), oil phase (oleic
acid) and emulsifying
agent (Tween 80: Span 80). Effect of
concentration of gelling agent on release of drug from transdermal delivery was
studied by 32 factorial design. Emulgel was evaluated for physical
appearance, pH, drug content, viscosity, spreadability, antimicrobial activity,
in- vitro diffusion study and ex-vivo diffusion
study.
Results:
FE-SEM
study of the emulsion batch B5 has revealed formation of emulsion globules of
approximately size 6-8 µm with -11.2 mV zeta potential showing good stability for the emulsion. Carbopol 940 had shown greater linear effect on drug
release and viscosity of the formulations due to its high degree of gelling. In-vitro diffusion study through egg membrane had
shown 88.58±1.82 % drug release for optimized batch F4. Ex-vivo diffusion study through goat skin indicated
76.68 ± 2.52% drug release.
Conclusion: Controlled release Ofloxacin
emulgel exhibiting good in-vitro and ex-vivo drug release proving good antimicrobial
property was formulated.