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Adv Pharm Bull. 2017;7(3): 479-483.
doi: 10.15171/apb.2017.058
PMID: 29071232
PMCID: PMC5651071
Scopus ID: 85030115718
  Abstract View: 2335
  PDF Download: 1721

Short Communication

Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria

Mahsa Esmaeillou 1, Gholamreza Zarrini 1*, Mohammad Ahangarzadeh Rezaee 2, Javid Shahbazi mojarrad 3, Ali Bahadori 4

1 Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
2 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Department of Medical Microbiology, Sarab Faculty of Medical Sciences, Sarab, Iran.
*Corresponding Author: Email: Zarrini@tabrizu.ac.ir

Abstract

Purpose: Many antimicrobial medications are available to combat infections. However, the indiscriminate use of antibiotics has produced antibiotic resistance in the case of many bacterial pathogens. This study focuses on the development of nanoparticles (NPs) that enhance the in vitro antibiotic activity of vancomycin against multi-drug resistant (MDR) organisms. Methods: Spherical shaped thioglycolic acid-stabilized silver nanoparticles (TGA-AgNPs) were prepared by using a simple chemical reduction method. Then, vancomycin was conjugated to the terminal carboxyl of TGA in the presence of N-Hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC). Afterwards, the antibacterial activity of these nanoconjugates was examined by using the minimum inhibitory concentration (MIC) assay against MDR bacteria. Results: The rate of vancomycin bound to the AgNPs was 19.6%. The MIC values of vancomycin (Van)-capped AgNPs against tested pathogens were in the range of (3.2, 1.6, 0.8, 0.4, 0.2, 0.1, 0.05, and 0.025 µl/ml). The MIC was 0.1 µg/ml for VRE, MIC≤0.02 µg/ml for MRSE, and 0.05 µg/ml for S. aureus. The MIC corresponded to the MBC for all bacterial species. Conclusion: This study indicated that some antimicrobial agents like vancomycin can be conjugated with AgNPs. This can lead to increased antimicrobial activity against MDR microorganisms.


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Submitted: 11 Feb 2017
Revision: 14 May 2017
Accepted: 05 Aug 2017
ePublished: 25 Sep 2017
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