Fatemeh Madani
1,2, Seyedeh Sara Esnaashari
1, Basil Mujokoro
3, Farid Dorkoosh
4, Masood Khosravani
1, Mahdi Adabi
1*1 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2 Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
4 Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
Purpose: The size
of polymeric nanoparticles is considered as an effective factor in cancer
therapy due to enterance into tumor tissue via the EPR effect. The purpose of this work was to investigate the
effective parameters on poly(lactic-co-glycolic
acid)-paclitaxel (PLGA –PTX) nanoparticles size.
Methods: We prepared PLGA-PTX nanoparticles via
single emulsion and precipitation methods with variable paremeters
including drug concentration, aqueous to organic
phase volume ratio, polymer concentration, sonication time and PVA
concentration.
Results: PLGA-PTX
nanoparticles were characterized by dynamic light scattering (DLS) and scanning
electron microscopy (SEM). The results exhibited that the diameter of
nanoparticles enhanced with increasing drug, polymer and PVA concentrations
whereas organic to aqueous phase volume ratio
and sonication time required to the optimization for a given size.
Conclusion: The precipitation method provides smaller nanoparticles compared to
emulsion one. Variable parameters including drug concentration, aqueous to organic phase volume ratio, polymer
concentration, sonication time and PVA concentration affect diameter of nanoparticles.