Azam Safary
1,2, Rezvan Moniri
1,3*, Maryam Hamzeh-Mivehroud
2,4, Siavoush Dastmalchi
2,4*1 Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.
2 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
4 School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Purpose: Robust pharmaceutical and industrial enzymes from extremophile
microorganisms are main source of enzymes with tremendous stability under harsh
conditions which make them potential tools for commercial and biotechnological
applications.
Methods: The genome of a Gram-positive halo-thermotolerant Bacillus
sp. SL1, new isolate from Saline Lake, was investigated for the presence of
genes coding for potentially pharmaceutical enzymes. We determined gene sequences for the enzymes laccase
(CotA), l-asparaginase (ansA3, ansA1), glutamate-specific endopeptidase
(blaSE), l-arabinose isomerase (araA2), endo-1,4-β mannosidase (gmuG),
glutaminase (glsA), pectate lyase (pelA), cellulase (bglC1), aldehyde
dehydrogenase (ycbD) and allantoinases (pucH) in the genome of Bacillus
sp. SL1.
Results: Based on the DNA sequence alignment results, six of
the studied enzymes of Bacillus sp. SL-1 showed 100% similarity at the
nucleotide level to the same genes of B. licheniformis 14580
demonstrating extensive organizational relationship between these two strains.
Despite high similarities between the B. licheniformis and Bacillus
sp. SL-1 genomes, there are minor differences in the sequences of some enzyme.
Approximately 30% of the enzyme sequences revealed more than 99% identity with some
variations in nucleotides leading to amino acid substitution in protein
sequences.
Conclusion: Molecular characterization
of this new isolate provides useful information regarding evolutionary
relationship between B. subtilis and
B. licheniformis species. Since, the most industrial processes are
often performed in harsh conditions, enzymes from such halo-thermotolerant
bacteria may provide economically and industrially appealing biocatalysts to be
used under specific physicochemical situations in medical, pharmaceutical,
chemical and other industries.