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Adv Pharm Bull. 2017;7(1): 159-164.
doi: 10.15171/apb.2017.020
PMID: 28507950
PMCID: PMC5426729
Scopus ID: 85017532615
  Abstract View: 1993
  PDF Download: 1214

Short Communication

Preparation and Characterization of Pistacia khinjuk Gum Nanoparticles Using Response Surface Method: Evaluation of Its Anti-Bacterial Performance and Cytotoxicity

Ali Fattahi 1,2,3, Tahereh Sakvand 4, Marziyeh Hajialyani 3, Behzad Shahbazi 2, Mohammad Shakiba 5, Ahmad Tajehmiri 1, Ebrahim Shakiba 6*

1 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2 Nano Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3 Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
4 Department of Medicinal plants, Kermanshah Jahade-Daneshgahi, Institute of Higher Education, Kermanshah, Iran.
5 Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
6 Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
*Corresponding Author: Email: eshakiba@kums.ac.ir

Abstract

Purpose: This study aims to prepare a novel, natural nanoparticle (NP) as a drug carrier, which also has inherent therapeutic effects.

Methods: Pistacia khinjuk gum NPs were prepared and Response surface methodology (RSM) was used for statistical analysis of data and optimizing the size of NPs.

Results: NPs were in the range of 75.85–241.3 nm. The optimization study was carried out, and an optimized size (70.86nm) was obtained using DMSO as a solvent. The volume of the organic phase was 111.25µl, and the concentration of gum was 1% w/v. The cell viability assay was performed on the pure gum and NPs toward β-TC3, MCF7, and HT29 cell lines. It was observed that NPs have higher cytotoxic activity in comparison with pure gum, and that the IC50value was achieved at 1% of NPs in β-TC3 cells. The obtained NPs demonstrated antibacterial activity against two bacterial strains (Pseudomonas aeruginosa and Staphylococcus aureus).

Conclusion: Altogether, according to the obtained results, these NPs with inherent cytotoxicity and antibacterial activity are an attractive carrier for drug delivery.



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Submitted: 01 Aug 2016
Revision: 15 Mar 2017
Accepted: 18 Mar 2017
ePublished: 13 Apr 2017
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