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Adv Pharm Bull. 2019;9(1): 147-158.
doi: 10.15171/apb.2019.018
PMID: 31011569
PMCID: PMC6468233
Scopus ID: 85066122321
  Abstract View: 405
  PDF Download: 406

Research Article

Enantioselective Box Behenken Optimized HPLC-DAD Method for the Simultaneous Estimation of Alogliptin Enantiomorphs in Pharmaceutical For mulations and their Pharmacokinetic Study in Rat Plasma

Ravi Kant 1 * ORCID logo, Ramesh Babu Bodla 1, Rubina Bhutani 1 ORCID logo, Garima Kapoor 1 ORCID logo

1 Pharmaceutical Chemistry Department, Delhi Institute of Pharmaceutical Sciences & Research, University of Delhi, Sector 3 Pushp Vihar, Mehrauli Badarpur Road, New Delhi -110017, India.

Abstract

Purpose: A stereoselective high performance liquid chromatographic analytical method withphotodiode array detector was developed and validated as per the International Conferenceon Harmonization (ICH) guidelines for the determination of alogliptin (ALO) enantiomers informulations and rat plasma.Methods: Enantiomeric separation was performed on a Phenomenex Lux Cellulose-2 chiralcolumn. Box-Behnken design was used to identify the optimum conditions of the threeindependent variables for the desired output responses.Results: The HPLC peaks of ALO enantiomers and the internal standard pioglitazone wereachieved before 8 min with a resolution of 0.77 min between R and S enantiomer and resolutionof more than 2.0 between each enantiomer and pioglitazone (internal) with more than 95%recovery. The linearity range and the limit of quantification of both the enantiomers in rat plasmawere 10-70 ng mL-1 and 1.2 ng mL-1 respectively.Conclusion: The developed method after validation was successfully applied for estimation ofALO enantiomers in formulations. Single oral dose of 25 mg of the ALO racemate tablets wereadministered to a group of 6 healthy rats for a comparative pharmacokinetic study of both theenantiomers.
Keywords: Alogliptin enantiomers, Box–Behnken Design, Pharmacokinetics, HPLC-DAD, SPE
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Submitted: 06 Jun 2018
Revision: 02 Oct 2018
Accepted: 20 Dec 2018
ePublished: 21 Feb 2019
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