Adv Pharm Bull. 2019;9(1): 147-158.
doi: 10.15171/apb.2019.018
PMID: 31011569
PMCID: PMC6468233
Scopus ID: 85066122321
  Abstract View: 680
  PDF Download: 572

Research Article

Enantioselective Box Behenken Optimized HPLC-DAD Method for the Simultaneous Estimation of Alogliptin Enantiomorphs in Pharmaceutical For mulations and their Pharmacokinetic Study in Rat Plasma

Ravi Kant 1* ORCID logo, Ramesh Babu Bodla 1, Rubina Bhutani 1 ORCID logo, Garima Kapoor 1 ORCID logo

1 Pharmaceutical Chemistry Department, Delhi Institute of Pharmaceutical Sciences & Research, University of Delhi, Sector 3 Pushp Vihar, Mehrauli Badarpur Road, New Delhi -110017, India.


Purpose: A stereoselective high performance liquid chromatographic analytical method withphotodiode array detector was developed and validated as per the International Conferenceon Harmonization (ICH) guidelines for the determination of alogliptin (ALO) enantiomers informulations and rat plasma.Methods: Enantiomeric separation was performed on a Phenomenex Lux Cellulose-2 chiralcolumn. Box-Behnken design was used to identify the optimum conditions of the threeindependent variables for the desired output responses.Results: The HPLC peaks of ALO enantiomers and the internal standard pioglitazone wereachieved before 8 min with a resolution of 0.77 min between R and S enantiomer and resolutionof more than 2.0 between each enantiomer and pioglitazone (internal) with more than 95%recovery. The linearity range and the limit of quantification of both the enantiomers in rat plasmawere 10-70 ng mL-1 and 1.2 ng mL-1 respectively.Conclusion: The developed method after validation was successfully applied for estimation ofALO enantiomers in formulations. Single oral dose of 25 mg of the ALO racemate tablets wereadministered to a group of 6 healthy rats for a comparative pharmacokinetic study of both theenantiomers.
Keywords: Alogliptin enantiomers, Box–Behnken Design, Pharmacokinetics, HPLC-DAD, SPE
First Name
Last Name
Email Address
Security code

Abstract View: 681

Your browser does not support the canvas element.

PDF Download: 572

Your browser does not support the canvas element.

Submitted: 06 Jun 2018
Revision: 02 Oct 2018
Accepted: 20 Dec 2018
ePublished: 21 Feb 2019
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)