Adv Pharm Bull. 2020;10(3): 430-436.
doi: 10.34172/apb.2020.052
  Abstract View: 60
  PDF Download: 66

Research Article

Grape Seed Oil as a Natural Therapy in Male Rats with Alzheimer’s Diseases

Farnoosh Berahmand 1 ORCID logo, Golnoush Anoush 2 ORCID logo, Mir-Jamal Hosseini 3 ORCID logo, Mahdieh Anoush 4 * ORCID logo

1 Student’s Research Center, Zanjan University of Medical Sciences, Zanjan, 4513956184, Iran.
2 Department of Toxicology, School of Pharmacy, Azad University of Shahreza, Isfahan, 8614510311, Iran.
3 Applied Pharmacology Research Center, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, 4513956184, Iran.
4 Department of Pharmacology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, 4513956184, Iran.


Purpose: Alzheimer’s disease (AD) is a chronic neurodegenerative disorder, with an increasingprevalence rate, mostly related to cholinergic system. According to the difficulties andcomplications in management of AD, this study was carried out to evaluate the efficacy of grapeseed oil (GSO) on scopolamine (Scop) induced Alzheimer’s in male rats.Methods: 64 healthy male Wistar rats received different treatments such as: normal saline (NS),donepezil (Don), Scop and GSO, according to the previously designed protocol. Morris (MWM)was applied for spatial memory tests. Right after the behavioral tests, the brains were removedand the hippocampus was separated for evaluation of acetylcholine levels as well as cell deathand neuro inflammation.Results: The results of the test day indicated that the mean Q2 time was increased in bothGSO test groups (P < 0.05) and Don treated group (P < 0.001).The spectrophotometric findingsaffirm that both GSO co-treatment and post-treatment were effective in augmenting brainacetylcholine levels (P < 0.01 and P < 0.05 respectively). The microscopic findings of H&E dyedtissues confirmed the above mentioned results for different treatments except for GSO posttreatment, in which the viability of cells were very low.Conclusion: The results implied that supplementation of rats with GSO caused a significantaugmentation in spatial memory performance as well as acetylcholine levels and cell viabilityin the presence of Scop. This effect was comparable to that of Don especially when GSO wasused as co-treatment.
Keywords: Acetylcholine, Alzheimer’s disease, Grape seed oil, Rat, Scopolamine, Spatial memory
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Submitted: 01 Dec 2018
Revision: 14 Nov 2019
Accepted: 23 Jan 2020
ePublished: 11 May 2020
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