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Adv Pharm Bull. 2019;9(3): 401-408.
doi: 10.15171/apb.2019.047
PMID: 31592118
PMCID: PMC6773926
Scopus ID: 85070460265
  Abstract View: 371
  PDF Download: 304

Research Article

Nanoemulsions of Green Tea Catechins and Other Natural Compounds for the Treatment of Urinary Tract Infection: Antibacterial Analysis

Atinderpal Kaur 1 ORCID logo, Reema Gabrani 1 ORCID logo, Shweta Dang 1 * ORCID logo

1 Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Noida, U.P., 201309, India.

Abstract

Purpose: Nanoemulsions (NEs) of polyphenon 60 (P60) and cranberry (NE I) and P60 and curcumin (NE II) were prepared with the aim to enhance anti-bacterial potential and to understand the mechanism of anti-bacterial action of the encapsulated compounds.

Methods: To evaluate the antibacterial potential of the developed NE, microtiter biofilm formation assay was performed. The cytotoxicity analysis was done to assess the toxicity profile of the NEs. Further antibacterial analysis against uropathogenic strains was performed to check the developed NEs were effective against these strains.

Results: In microtiter dish biofilm formation assay, both NE formulations inhibited the growth more effectively (Av. % inhibition ~84%) as compared to corresponding aqueous solution (Av. % inhibition ~64%) and placebo (Av. % inhibition ~59%) at their respective minimum inhibitory concentration (MIC) values. Cytotoxicity analysis using 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT assay) showed that the formulations were nontoxic to Vero cells. The antibacterial studies against uropathogenic resistant strains also showed that NEs effectively inhibited the growth of bacterial strains.

Conclusion: From different studies it was concluded that both the NE’s were able to inhibit bacterial strains and could be further used for the treatment of urinary tract infection (UTI). The antibacterial activity of developed NEs showed that these could be used as alternative therapies for the treatment of UTI.

Keywords: Biofilm, Cranberry, Curcumin, Cytotoxicity, Green tea, Nanoemulsion
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Submitted: 14 Dec 2018
Revision: 07 May 2019
Accepted: 09 May 2019
ePublished: 01 Aug 2019
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