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Adv Pharm Bull. 2020;10(1): 39-45.
doi: 10.15171/apb.2020.005
PMID: 32002360
PMCID: PMC6983993
Scopus ID: 85084217586
  Abstract View: 490
  PDF Download: 336

Research Article

Development and Characterization of Nanoliposomal Hydroxyurea Against BT-474 Breast Cancer Cells

Azam Akbari 1,2 ORCID logo, Azim Akbarzadeh 2* ORCID logo, Morteza Rafiee Tehrani 1, Reza Ahangari Cohan 2 ORCID logo, Mohsen Chiani 2, Mohammad Reza Mehrabi 2

1 Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
2 Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.

Abstract

Purpose: Hydroxyurea (HU) is a well-known chemotherapy drug with several side effects which limit its clinical application. This study was conducted to improve its therapeutic efficiency against breast cancer using liposomes as FDA-approved drug carriers.

Methods: PEGylated nanoliposomes-containing HU (NL-HU) were made via a thin-film hydration method, and assessed in terms of zeta potential, size, morphology, release, stability, cellular uptake, and cytotoxicity. The particle size and zeta potential of NL-HU were specified by zeta-sizer. The drug release from liposomes was assessed by dialysis diffusion method. Cellular uptake was evaluated by flow cytometry. The cytotoxicity was designated by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) test.

Results: The size and zeta value of NL-HU were gotten as 85 nm and -27 mV, respectively. NL-HU were spherical.NL-HU vesicles were detected to be stable for two months. The slow drug release and Weibull kinetic model were obtained. Liposomes considerably enhanced the uptake of HU into BT-474 human breast cancer cells. The cytotoxicity of NL-HU on BT-474 cells was found to be significantly more than that of free HU.

Conclusion: The results confirmed these PEGylated nanoliposomes containing drug are potentially suitable against in vitro model of breast cancer.

Keywords: Breast neoplasms, Drug carriers, Hydroxyurea, Liposomes
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Submitted: 24 Feb 2019
Revision: 13 Sep 2019
Accepted: 18 Sep 2019
ePublished: 11 Dec 2019
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