Adv Pharm Bull. 2019;9(3): 416-422.
doi: 10.15171/apb.2019.049
PMID: 31592074
PMCID: PMC6773932
Scopus ID: 85070445393
  Abstract View: 988
  PDF Download: 418

Research Article

Determination of Methyldopa and Paracetamol in Pharmaceutical Samples by a Low Cost Genipa americana L. Polyphenol Oxidase Based Biosensor

Rafael Souza Antunes 1, Douglas Vieira Thomaz 1* ORCID logo, Luane Ferreira Garcia 1, Eric de Souza Gil 1 ORCID logo, Vernon Sydwill Sommerset 2, Flavio Marques Lopes 1 ORCID logo

1 Faculdade de Farmácia, Universidade Federal do Goiás (UFG), rua 221 esquina com a 5ª avenida s/n, Setor Universitário, Goiânia-GO, Brasil.
2 Department of Chemistry, Faculty of Applied Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa.


Purpose: Jenipapo fruit (Genipa americana L) is a natural source of polyphenol oxidases (PPOs) whose potential in pharmaceutical analysis is noteworthy. Henceforth, this work reports the electrochemical study of a low-cost PPO-based biosensor produced from the crude extract of Jenipapo fruits and accounts a practical approach to employ this biosensor in the determination of methyldopa and paracetamol in pharmaceutical samples.

Methods: In order to investigate the electrochemical properties of the biosensor, theoretical and practical approaches were employed, and both samples and the biosensor were analyzed through electrochemical impedance spectroscopy (EIS) and voltammetric techniques, namely: differential pulse voltammetry (DPV) and cyclic voltammetry (CV).

Results: showcased that the biosensor presented good analytical features, as well as low detection limits (8 μmol L-1 for methyldopa and 5 μmol L-1 for paracetamol). The relative standard deviation was less than 5% mid-assay.

Conclusion: The use of this biosensor is a reliable, low cost and useful alternative in the pharmaceutic determination of phenolic drugs (e.g. methyldopa and paracetamol).

Keywords: Jenipapo (Genipa americana L.), Biosensor, Methyldopa, Paracetamol
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Submitted: 12 Mar 2019
Revision: 31 May 2019
Accepted: 03 Jun 2019
ePublished: 01 Aug 2019
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