Adv Pharm Bull. 2019;9(3):416-422.
doi: 10.15171/apb.2019.049
  Abstract View: 27
  PDF Download: 36

Research Article

Determination of Methyldopa and Paracetamol in PharmaceuticalSamples by a Low Cost Genipa americana L. Polyphenol OxidaseBased Biosensor

Rafael Souza Antunes 1, Douglas Vieira Thomaz 1 * ORCiD, Luane Ferreira Garcia 1, Eric de Souza Gil 1 ORCiD, Vernon Sydwill Sommerset 2, Flavio Marques Lopes 1 ORCiD

1 Faculdade de Farmácia, Universidade Federal do Goiás (UFG), rua 221 esquina com a 5ª avenida s/n, Setor Universitário, Goiânia-GO, Brasil.
2 Department of Chemistry, Faculty of Applied Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa. Introduction


Purpose: Jenipapo fruit (Genipa americana L) is a natural source of polyphenol oxidases (PPOs)whose potential in pharmaceutical analysis is noteworthy. Henceforth, this work reports theelectrochemical study of a low-cost PPO-based biosensor produced from the crude extract ofJenipapo fruits and accounts a practical approach to employ this biosensor in the determinationof methyldopa and paracetamol in pharmaceutical samples.Methods: In order to investigate the electrochemical properties of the biosensor, theoreticaland practical approaches were employed, and both samples and the biosensor were analyzedthrough electrochemical impedance spectroscopy (EIS) and voltammetric techniques, namely:differential pulse voltammetry (DPV) and cyclic voltammetry (CV).Results: showcased that the biosensor presented good analytical features, as well as lowdetection limits (8 μmol L-1 for methyldopa and 5 μmol L-1 for paracetamol). The relativestandard deviation was less than 5% mid-assay.Conclusion: The use of this biosensor is a reliable, low cost and useful alternative in thepharmaceutic determination of phenolic drugs (e.g. methyldopa and paracetamol).
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Submitted: 12 Mar 2019
Revised: 31 May 2019
Accepted: 03 Jun 2019
First published online: 01 Aug 2019
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