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Adv Pharm Bull. 2020;10(3): 482-487.
doi: 10.34172/apb.2020.060
  Abstract View: 57
  PDF Download: 46

Short Communication

Detoxification of Aflatoxin B1 by Probiotic Yeasts and Bacteria Isolated from Dairy Products of Iran

Hajar Zolfaghari 1 ORCID logo, Arezou Khezerlou 2 ORCID logo, Ali Ehsani 1,3 * ORCID logo, Ahmad Yari Khosroushahi 4,5 * ORCID logo

1 Department of Food Science and Technology, Nutrition and Food Science Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Student Research Committee, Department of Food Science and Technology, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Purpose: The present study was conducted to assess the ability of probiotic bacteria andyeasts strains to reduce aflatoxin B1 (AFB1) in gastrointestinal simulated conditions. Aflatoxinsare potent carcinogenic and immunosuppressive agents. Acute exposure to a high level ofaflatoxins leads to aflatoxicosis, which cause rapid death due to liver failure. It is anticipatedthat consumption of probiotic microorganisms capable of binding aflatoxins can reduce the riskof AFB1 on human health to a certain extent.Methods: For this purpose, the bacteria (1 × 1010 cfu/mL) and yeasts count (2 × 108 cells/mL) andAFB1 concentration (10 ppb) were adjusted. Then, the samples were incubated in the simulatedmedium, human gastric secretions and small intestine. The concentration of residual AFB1 wasdetermined using enzyme-linked immunosorbent assay (ELISA). The results were statisticallyanalyzed by SPSS 16 software.Results: The native isolated bacteria and yeasts in the simulated gastrointestinal tract conditionshowed a significant effect on AFB1 reduction (P < 0.05). The AFB1 reduction ability of nativeprobiotic microorganisms was strain dependent. The highest binding ability in bacteria belongedto Lactobacillus rhamnosus (31.14%) and at yeasts belonged to Saccharomyces cerevisiae(30.46%).Conclusion: The use of probiotic strains is the appropriate biological method to reduce AFB1 inthe human gastrointestinal tract. Probiotic bacteria could help to decrease the harmful effects ofAFB1 in humans through enhancing the food safety.
Keywords: Aflatoxin B1, Detoxification, ELISA, Food safety, Probiotic bacteria
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Submitted: 20 May 2019
Revision: 09 Nov 2019
Accepted: 02 Dec 2019
ePublished: 11 May 2020
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