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Adv Pharm Bull. 2020;10(3): 482-487.
doi: 10.34172/apb.2020.060
PMID: 32665910
PMCID: PMC7335981
Scopus ID: 85088617905
  Abstract View: 593
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Short Communication

Detoxification of Aflatoxin B1 by Probiotic Yeasts and Bacteria Isolated From Dairy Products of Iran

Hajar Zolfaghari 1 ORCID logo, Arezou Khezerlou 2 ORCID logo, Ali Ehsani 1,3* ORCID logo, Ahmad Yari Khosroushahi 4,5* ORCID logo

1 Department of Food Science and Technology, Nutrition and Food Science Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Student Research Committee, Department of Food Science and Technology, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
*Corresponding Authors: Ali Ehsani and Ahmad Yari Khosroushahi, Tel. +98 41 33363234, Fax. +98 41 33363231, Email:, Email: yarikhosroushahia@tbzmed.ac.ir ehsani@tbzmed. ac.ir

Abstract

Purpose: The present study was conducted to assess the ability of probiotic bacteria and yeasts strains to reduce aflatoxin B1 (AFB1) in gastrointestinal simulated conditions. Aflatoxins are potent carcinogenic and immunosuppressive agents. Acute exposure to a high level of aflatoxins leads to aflatoxicosis, which cause rapid death due to liver failure. It is anticipated that consumption of probiotic microorganisms capable of binding aflatoxins can reduce the risk of AFB1 on human health to a certain extent.

Methods: For this purpose, the bacteria (1 × 1010 cfu/mL) and yeasts count (2 × 108 cells/mL) and AFB1 concentration (10 ppb) were adjusted. Then, the samples were incubated in the simulated medium, human gastric secretions and small intestine. The concentration of residual AFB1 was determined using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by SPSS 16 software.

Results: The native isolated bacteria and yeasts in the simulated gastrointestinal tract condition showed a significant effect on AFB1 reduction (P<0.05). The AFB1 reduction ability of native probiotic microorganisms was strain dependent. The highest binding ability in bacteria belonged to Lactobacillus rhamnosus (31.14%) and at yeasts belonged to Saccharomyces cerevisiae (30.46%).

Conclusion: The use of probiotic strains is the appropriate biological method to reduce AFB1 in the human gastrointestinal tract. Probiotic bacteria could help to decrease the harmful effects of AFB1 in humans through enhancing the food safety.

Keywords: Aflatoxin B1, Detoxification, ELISA, Food safety, Probiotic bacteria
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Submitted: 20 May 2019
Revision: 09 Nov 2019
Accepted: 02 Dec 2019
ePublished: 11 May 2020
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