Adv Pharm Bull. 2021;11(3): 477-489.
doi: 10.34172/apb.2021.055
  Abstract View: 419
  PDF Download: 20

Research Article

Novel Methotrexate-Ciprofloxacin Loaded Alginate-Clay Based Nanocomposite as Anticancer and Antibacterial Co-Drug Delivery System

Mehrdad Mahkam 1 ORCID logo, Fatemeh Bazmi Zeynabad 1, Effat Alizadeh 2, Mahdi Rahimi 3, Fariborz Rahimi 4* ORCID logo, Roya Salehi 5* ORCID logo

1 Chemistry Department, Azarbaijan Shahid Madani University, Tabriz, Iran.
2 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
4 Department of Electrical Engineering, University of Bonab, Bonab, Iran.
5 Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.


Purpose: In last decades, by increasing multi-drug resistant microbial pathogens an urgent demand was felt in the development of novel antimicrobial agents.
Methods: Promising nanocomposites composed of clay/alginate/imidazolium-based ionic liquid, have been developed via intercalation of calcium alginate and ionic liquid by ion-exchange method. These tailored nanocomposites were used as nanocarriers to simultaneously deliver methotrexate (MTX), and ciprofloxacin (CIP), as anticancer and antibacterial agents, respectively to MCF-7 breast cancer cells. Nanocomposites were fully characterized by SEM, XRD, FTIR spectroscopy, and TGA methods. The in vitro antimicrobial potential of the mentioned nanocomposites in free and dual-drug loaded form was investigated on Pseudomonas aeruginosa and Escherichia coli bacteria. The antitumor activity of nano-formulations was evaluated by both MTT assay and cell cycle arrest.
Results: The dual drug-loaded nanocomposites with exceptionally high loading efficiency (MTX: 99 ±0.4% and CIP: 98 ±1.2%) and mean particle size of 70 nm were obtained with obvious pH-responsive MTX and CIP release (both drugs release rate was increased at pH 5.8 compared to 7.4). The antibacterial activity of CIP-loaded nanocomposites was significantly higher in comparison with free CIP (p <0.001). The antitumor activity results revealed that MTX cytotoxicity on MCF-7 cells was significantly higher in nano-formulations compared to free MTX (p <0.001). Both MTX-loaded nanocomposites caused S-phase arrest in MCF-7 cells compared to non-treated cells (P˂0.001).
Conclusion: Newly developed smart nanocomposites are potentially effective pH-sustainable delivery systems for enhanced tumor therapy.
Keywords: Drug delivery system, Alginate, Nanoclay, PH-sensitive nanocomposite, Cancer therapy, Antibacterial activity
First Name
Last Name
Email Address
Security code

Abstract View: 420

Your browser does not support the canvas element.

PDF Download: 20

Your browser does not support the canvas element.

Submitted: 14 Sep 2019
Revision: 22 May 2020
Accepted: 30 Jun 2020
ePublished: 01 Jul 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)