Logo-apb
Adv Pharm Bull. 2022;12(4): 828-834.
doi: 10.34172/apb.2022.084

Scopus ID: 85140416016
  Abstract View: 616
  PDF Download: 307
  Full Text View: 54

Research Article

Decreased Expression of LAMB3 Is Associated with EsophagealCancer Stem Cell Formation

Anoosheh Ehtesham 1 ORCID logo, Ayyoob Khosravi 2,3 ORCID logo, Marie Saghaeian Jazi 2 ORCID logo, Jahanbakhsh Asadi 1,2* ORCID logo, Seyyed Mehdi Jafari 1,4* ORCID logo

1 Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
2 Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
3 Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran.
4 Department of Biochemistry and Biophysics, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
*Corresponding Authors: Corresponding Author: Jahanbakhsh Asadi, Email: , Email: dr.asadi@goumc.ac.ir; Corresponding Author: Seyyed Mehdi Jafari, Email: , Email: s.meh.jafari@goumc.ac.ir

Abstract

Purpose: Esophageal squamous cell carcinoma (ESCC) is a highly aggressive cancer. The maincause of death in ESCC is related to relapse, metastasis, and resistance to cancer therapy. Recentstudies have shown that a minor subset of cancer cells, known as cancer stem cells (CSCs), areresponsible for tumor formation initiation and cancer progression. Understanding the genesassociated with CSCs and metastasis can help in targeted cancer therapy. The aim of this studywas to assess the expression of LAMB3 and TOP2A metastasis-associated genes in CSCs andadherent cells in the xenograft mouse model.Methods: Esophageal CSCs were enriched by the sphere formation method. The expressionlevel of LAMB3 and TOP2A genes were evaluated in spheres and adherent cells in vitro byqRT-PCR. A xenograft mouse model was established to investigate the tumorigenesis andmetastasis potential by subcutaneous and tail vein injection of CSCs and adherent YM-1 cells.Consequently, LAMB3 and TOP2A expression at the mRNA level was assessed in tumors.Immunohistochemistry was also used to evaluate the LAMB3 expression at the protein levelin tumors.Results: CSCs-derived tumor was developed more quickly than the adherent cells-derivedtumor. LAMB3 at mRNA and protein level was significantly down-regulated in sphere-derivedtumor compared with adherent cells-derived tumor (P value <0.05). TOP2A expression wasalmost similar in both sphere cells and adherent cells and there was no significant difference.Conclusion: we concluded that YM-1 spheres have CSCs characteristics in vitro with highcapability of tumorigenicity in vivo. Our results were also shown that the LAMB3 expressionwas decreased in YM-1 spheres suggesting LAMB3 association with sphere formation.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 617

Your browser does not support the canvas element.


PDF Download: 307

Your browser does not support the canvas element.


Full Text View: 54

Your browser does not support the canvas element.

Submitted: 01 Aug 2020
Revision: 07 May 2021
Accepted: 27 Sep 2021
ePublished: 29 Sep 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)