Logo-apb
Adv Pharm Bull. 2023;13(4): 736-746.
doi: 10.34172/apb.2023.072
  Abstract View: 527
  PDF Download: 471

Research Article

17β-Estradiol-Loaded Exosomes for Targeted Drug Delivery in Osteoporosis: A Comparative Study of Two Loading Methods

Mohammad Sadegh Gholami Farashah 1,2,3,4 ORCID logo, Maryam Javadi 3,4, Jafar Soleimani Rad 3,4, Seyed Kazem Shakouri 1, Solmaz Asnaashari 2, Siavoush Dastmalchi 2,5,6, Sadeneh Nikzad 7, Leila Roshangar 1,3,4* ORCID logo

1 Physical Medicine and Rehabilitation Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Department of Anatomical Sciences, Faculty of medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Faculty of Pharmacy, Near East University, POBOX:99138, Nicosia, North Cyprus, Mersin 10, Turkey.
7 Biology Department, Concordia University, Montreal, Canada.
*Corresponding Author: Department of Anatomical Sciences, Faculty of medicine, Tabriz University of Medical Sciences, Tabriz, Iran Email lroshangar@yahoo.com
*Corresponding Author: Leila Roshangar, Email: lroshangar@yahoo.com

Abstract

Purpose: Exosomes are natural nanoparticles that participate in intercellular communication through molecular transport. Recently, due to their membrane vesicular structure and surface proteins, exosomes have been used extensively in the research field of drug delivery. Osteoporosis is an inflammation in which the cellular balance of bone tissue is disturbed that reduces bone density and making bone prone to abnormal fractures with small amount of force. Utilizing estrogen is one of the main therapeutic strategies for osteoporosis. Despite the positive effects of estrogen on bone tissue, changes in the natural estrogen levels of the body can cause a number of diseases such as different types of cancer. Therefore, designing a therapeutic system which controls more accurate tissue targeting of estrogen seems to be a rational and promising practical approach.

Methods: In this study, bone marrow mesenchymal stem cells (BMMSCs)-derived exosomes were loaded by estradiol using two different methods of drug loading, namely incubation and sonication methods and then the survival effects of the drug loaded exosomes on BMMSCs was investigated.

Results: Examination of size, shape, and surface factors of exosomes in different states (pure exosomes and drug-loaded exosomes) showed that the round morphology of exosomes was preserved in all conditions. However, the particles size increased significantly when loaded by sonication method. The increased survival of BMMSCs was noted with estradiol-loaded exosomes when compared to the control group.

Conclusion: The results suggest that estradiol-loaded exosomes have potential to be used as nano-drug carriers in the treatment of osteoporosis.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 528

Your browser does not support the canvas element.


PDF Download: 471

Your browser does not support the canvas element.

Submitted: 22 Oct 2022
Revision: 23 Jan 2023
Accepted: 30 Jan 2023
ePublished: 01 Feb 2023
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)