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Adv Pharm Bull. 2019;9(3): 481-489.
doi: 10.15171/apb.2019.057
PMID: 31592119
PMCID: PMC6773930
Scopus ID: 85070472005
  Abstract View: 367
  PDF Download: 302

Research Article

Evaluation of the Cytotoxic and Apoptogenic Effects of Glabridin and Its Effect on Cytotoxicity and Apoptosis Induced by Doxorubicin Toward Cancerous Cells

Masoud Modarresi 1 ORCID logo, Marziyeh Hajialyani 1 ORCID logo, Narges Moasefi 2 ORCID logo, Farahnaz Ahmadi 1 ORCID logo, Leila Hosseinzadeh 1 * ORCID logo

1 Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, 6734667149, Iran.
2 Medical Biology Research Center , Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract

Purposes: In the present study, we tried for the first time to examine the anti-proliferative and anti-apoptogenic effect of Glabridin (Glab) toward three groups of cancer cells (SKNMC, H1299, and A2780). Furthermore, the possibility of co-administration of Glab with doxorubicin (DOX) to these cells was also examined to find out whether Glab can potentiate the cytotoxic effect of this chemotherapy agent.

Methods: Different cellular assays (MTT, caspase-3 activity, MMP, RT-PCR analysis) were carried out on the cancer cells treated with Glab.

Results: Cellular toxicity assay revealed that Glab can potentially reduce the viability of these cells with IC50 concentrations up to 10, 12, and 38 μM toward A2780, SKNMC, and H1299 cell lines, respectively. The results of MMP and caspase-3 activity assays, in association with the results corresponding to the BAX and Bcl-2 gene expressions, altogether revealed that Glab can exert apoptogenic effect on these cells. The intrinsic mitochondrial pathway was found to be the main mechanism, in which Glab induced apoptosis toward H1299 cells and SKNMC cells, while the apoptosis mechanism for A2780 cells could be probably through extrinsic pathway. Glab also potentiated the cytotoxic effect of DOX and its accumulation in H1299 cell line.

Conclusion: The results of this study revealed the promising cytotoxic role of Glab on different carcinoma cells. These data also suggested that co-chemotherapy method using Glab could be effective for treatment of cancer, but further in-vivo and clinical studies are still needed to assure these results.

Keywords: Apoptosis, Cytotoxicity, Doxorubicin, Glabridin, Herbal medicine, Licorice
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Submitted: 30 Jan 2019
Revision: 18 Apr 2019
Accepted: 04 May 2019
ePublished: 01 Aug 2019
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