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Adv Pharm Bull. 2019;9(3): 490-496.
doi: 10.15171/apb.2019.058
PMID: 31592099
PMCID: PMC6773927
Scopus ID: 85070483360
  Abstract View: 339
  PDF Download: 263

Research Article

The Effects of Thymoquinone on Viability, and Anti-apoptotic Factors (BCL-XL, BCL-2, MCL-1) in Prostate Cancer (PC3) Cells: An In Vitro and Computer-Simulated Environment Study

Javad Saffari_Chaleshtori 1 ORCID logo, Ehsan Heidari-Sureshjani 2, Fahimeh Moradi 3, Esfandiar Heidarian 4 * ORCID logo

1 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
2 Young Researchers and Elites Club, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
3 Cellular & Molecular, Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
4 Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Abstract

Purpose: Since active plant ingredients can induce apoptosis in many tumors, in this study we evaluate the apoptotic effects of thymoquinone (TQ) on PC3 cells. Also, we predicted the interaction of TQ with BCL-XL, BCL-2, and MCL-1anti-apoptotic factors by computer-simulated environment.

Methods: PC3 cells were treated with different concentrations of TQ (0- 80 µM) and IC50 was determined using 3-(4, 5-dimethylthiaztol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Apoptotic and cytotoxicity effects of TQ were analyzed using flowcytometry and comet assay, respectively. Changes in energy and the molecular interactions of TQ with BCL-XL, BCL-2 and MCL-1 anti-apoptotic factors were investigated using simulation.

Results: IC50 value was 40 µM. TQ led to the destruction of the genome of PC3 cells and inducing apoptosis. Molecular dynamics (MD) revealed that the root mean-square deviation (RMSD), root mean square fluctuation (RMSF), radius of gyration (Rg), and the number of hydrogen and hydrophobic bonds between TQ and residues of BCL-2, BCL-XL and MCL-1were significantly (P<0.001) changed. TQ makes a more stable and stronger connection with BCL-XL compared to BCL-2 and MCL-1 and inhibits BCL-XL non-competitively.

Conclusion: Our results demonstrated that TQ not only led to apoptosis, at least partly, due to reduction in the Coil, Turn, and Bend structure of BCL-XL but also caused a decrease in the Rg and RMSD value of BCL-XL, MCL-1, and BCL-2.

Keywords: Thymoquinone, Apoptosis, Cancer, Simulation
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Submitted: 10 Feb 2019
Revision: 18 Mar 2019
Accepted: 14 Apr 2019
ePublished: 01 Aug 2019
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